![]() ![]() Seeds were stored at 4☌ in commercial packs until they were used in experiments. The seeds of onion ( Allium cepa L.) (2 n = 16) of the Stuttgarter Risen variety were used in the experiment. seedlings in the long terms after irradiation. The aim of this work is to evaluate the effect of γ-radiation on cytogenetic and growth parameters of onion Allium cepa L. ![]() For an objective assessment of the long-term effects of γ-irradiation in experiments, long-term observations of not only the growth but also the cytogenetic parameters of onion seedlings after irradiation are required. However, the authors provide data on the levels of cytogenetic disorders only at the initial moment of plant growth (0 and 20 h after irradiation). According to the authors, the inhibition of plant growth in the second generation can be explained by genomic instability induced by irradiation. , when pea seedlings were irradiated at doses up to 10 Gy, inhibitory effects on plant growth were noted, which were recorded after several generations. Many authors do not suggest the mechanisms of inhibition of the growth of irradiated plants. the growth of wheat roots was inhibited at doses above 30 Gy. , plant growth inhibition was observed at radiation doses from 5 to 50 Gy and higher, in the study by Liu et al. For example, in the review by Gudkov et al. A number of authors described the inhibition of plant growth parameters after irradiation however, they used high doses. The absence of inhibition of growth processes can be explained by both relatively low doses of γ-irradiation and the short duration of our experiments (24 h). However, in our experiments, at a high level of chromosome aberrations, no effect of radiation on root growth was revealed. Previously, it was noted that DNA damage in the cells of living organisms can lead to termination of their growth, as well as to long-term consequences. At the maximum absorbed dose of 13 Gy, chromosome aberrations were observed in the majority of onion cells at the anaphase and telophase stages of mitosis, and the number of cells with multiple disorders increased. For onion seedlings, an increase in the frequency of cytogenetic disorders in cells was found at all doses of γ-irradiation for 24 h, including low doses. In toxicological experiments, we used the onion biotest ( Allium-test), which has shown itself well in assessing the toxicity of environmental samples. Previously, we performed laboratory experiments with various bioassays to simulate the effect of γ-radiation of radioactive particles and showed the high sensitivity of bioassays to γ-radiation. Hydrobionts of the Yenisei River experience an additional dose load due to technogenic radionuclides, including those in the form of radioactive microparticles, as a result of long-term industrial discharges of the Mining and Chemical Combine of the State Corporation Rosatom into the river. Nuclear weapons testing and the activities of nuclear fuel cycle enterprises have led to the release of a significant amount of technogenic radionuclides into the environment. ![]()
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